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KMID : 0380020070220060371
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Korean Journal of Biotechnology and Bioengineering
2007 Volume.22 No. 6 p.371 ~ p.377
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A Research and Application of Polyhydroxyalkanoates in Biosensor Chip
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Lee Sang-Yup
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Abstract
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Polyhydroxyalkanoates (PHAs) are a family of microbial polyesters that can be produced by fermentation from renewable resources. PHAs can be used as completely biodegradable plastics or elastomers. In this paper, novel applications of PHAs in biosensor are described. A general platform technology was developed by using the substrate binding domain (SBD) of PHA depolymerase as a fusion partner to immobilize proteins of interest on PHA surface. It could be shown that the proteins fused to the SBD of PHA depolymerase could be specifically immobilized onto PHA film, PHA microbead, and microcontact printed PHA surface. We review the results obtained for monitoring the specific interaction between the SBO and PHA by using enhanced green fluorescent protein, red fluorescent protein, single chain antibody against hepatitis B virus preS2 surface protein and severe acute respiratory syndrome coronavirus surface antigen as model proteins. Thus, this system can be efficiently used for studying protein-protein and possibly protein-biomolecule interactions for various biotechnological applications.
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KEYWORD
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Poly(3-hydroxybutyrate), P(3HB) depolymerase, substrate binding domain, protein microarray
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